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Servicing: Microbiological Safety Cabinets

A Safe Working Area

A class II Cabinet or a microbiological safety cabinet is a piece of laboratory equipment that provides a safe working area for people handling material potentially contaminated with pathogens.

Principle of Operation

The principle of operation involves using a fan mounted in the top of the cabinet to draw a curtain of sterile air over the products that are being handled. The air is then drawn underneath the work surface and back up to the top of the cabinet where it passes through filters (HEPA filters) that are capable of removing harmful bacteria and viruses. Seventy percent of the air is continuously re-circulated in this way, while 30 percent is exhausted through another HEPA filter in the top of the cabinet.

The air that is exhausted is made up by air being drawn into the front of the cabinet underneath the work surface. The air being drawn in acts as a barrier to potentially contaminated air coming back out to the operator.

For your safety and the safety of laboratory personnel, it is important that a full safety check and testing of your microbiological safety cabinet is completed as often as deemed necessary.

Performance Tests

Performance tests are carried out to BS EN12469. Included in the testing and validation of your microbiological safety cabinet is:

• The operation of airflow arms and indicators
• Airflow velocity
• Visualisation tests and particle count tests
• HEPA filter integrity and
• Containment testing

In compliance with health and safety regulations, microbiological safety cabinets used for known and potential human pathogens must be rendered safe before use.

NSP Laboratory Services Ltd. supply a full range of filter replacements for Microbiological Safety Cabinets.

Contact NSP Laboratory Services Ltd.

Contact NSP Laboratory Services Ltd. for further information about Microbiological Safety Cabinets.